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Sarstedt suspension cell culture flasks
Suspension Cell Culture Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/suspension+cell+culture+flasks/cell+culture+flasks/pm41913181-72-9-13
Average 86 stars, based on 1 article reviews
suspension cell culture flasks - by Bioz Stars, 2026-09
86/100 stars

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Cell Culture:

Article Title: Glioblastoma-natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis.
Article Snippet: .. Both cell lines were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: Glioblastoma–natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis
Article Snippet: .. Both cell lines were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B-27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors
Article Snippet: Primary T cell medium was also supplemented with Interleukin-2 (IL-2, research grade, Miltenyi Biotec, Surrey, UK) as specified. .. Cells were thawed at 2x10 6 cells/mL in T cell complete medium in suspension cell culture flasks (Sarstedt AG & Co. KG, Germany). .. One day after thaw, the T cells were activated at 1:1 ratio of cells to Magnetic Dynabeads ® (Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer protocol with a final cell density of 1x10 6 cells/mL.

Article Title: Glioblastoma–natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis
Article Snippet: GB stem-like cell line NCH421k was purchased from Cell Lines Service GmbH (CLS). .. Cells were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B-27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca 2+ microdomains to initiate T cell activation.
Article Snippet: Kai J. Winterberg†, Vanessa Schwentner†, Feng Gu, Franziska Möckl, Gaoyang Li, Andreas Bauche, Stefanie Etzold, Anette Rosche, Mariella Weiß, Nikolaus Thuille, Fritz Förster, Lena Woelk, René Werner, Dejan Kovacevic, Boris Fehse, Roberta Kurelic, Mikolaj Nawrocki, Samuel Huber, HansWilli Mittrücker, Chris Meier, Christa E. Müller, Gottfried Baier, Bjørn S. Skålhegg, Xavier de Deken, Christian WahlSchott, Thomas Mair, Bente Siebels, Roger Cugota Canals, Francesca Odoardi, Dmitri Lodygin, Alexander Flügel, Viacheslav O. Nikolaev, BjörnPhilipp Diercks, Andreas H. Guse*

Suspension:

Article Title: Glioblastoma-natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis.
Article Snippet: .. Both cell lines were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: Glioblastoma–natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis
Article Snippet: .. Both cell lines were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B-27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors
Article Snippet: Primary T cell medium was also supplemented with Interleukin-2 (IL-2, research grade, Miltenyi Biotec, Surrey, UK) as specified. .. Cells were thawed at 2x10 6 cells/mL in T cell complete medium in suspension cell culture flasks (Sarstedt AG & Co. KG, Germany). .. One day after thaw, the T cells were activated at 1:1 ratio of cells to Magnetic Dynabeads ® (Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer protocol with a final cell density of 1x10 6 cells/mL.

Article Title: Glioblastoma–natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis
Article Snippet: GB stem-like cell line NCH421k was purchased from Cell Lines Service GmbH (CLS). .. Cells were cultured as floating spheres in suspension cell culture flasks (Sarstedt) in Neurobasal Medium (NBE; Gibco, Thermo Fisher Scientific) supplemented with 1× penicillin/streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), 1× B-27 (Invitrogen), 1 U/mL heparin (Sigma-Aldrich), 20 ng/mL basic fibroblast growth factor (Invitrogen) and 20 ng/mL epidermal growth factor (Invitrogen). .. Spheres were regularly dissociated with TrypLE Express (Gibco, Thermo Fisher Scientific).

Article Title: Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca 2+ microdomains to initiate T cell activation.
Article Snippet: Kai J. Winterberg†, Vanessa Schwentner†, Feng Gu, Franziska Möckl, Gaoyang Li, Andreas Bauche, Stefanie Etzold, Anette Rosche, Mariella Weiß, Nikolaus Thuille, Fritz Förster, Lena Woelk, René Werner, Dejan Kovacevic, Boris Fehse, Roberta Kurelic, Mikolaj Nawrocki, Samuel Huber, HansWilli Mittrücker, Chris Meier, Christa E. Müller, Gottfried Baier, Bjørn S. Skålhegg, Xavier de Deken, Christian WahlSchott, Thomas Mair, Bente Siebels, Roger Cugota Canals, Francesca Odoardi, Dmitri Lodygin, Alexander Flügel, Viacheslav O. Nikolaev, BjörnPhilipp Diercks, Andreas H. Guse*

Knockdown:

Article Title: Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca 2+ microdomains to initiate T cell activation.
Article Snippet: Kai J. Winterberg†, Vanessa Schwentner†, Feng Gu, Franziska Möckl, Gaoyang Li, Andreas Bauche, Stefanie Etzold, Anette Rosche, Mariella Weiß, Nikolaus Thuille, Fritz Förster, Lena Woelk, René Werner, Dejan Kovacevic, Boris Fehse, Roberta Kurelic, Mikolaj Nawrocki, Samuel Huber, HansWilli Mittrücker, Chris Meier, Christa E. Müller, Gottfried Baier, Bjørn S. Skålhegg, Xavier de Deken, Christian WahlSchott, Thomas Mair, Bente Siebels, Roger Cugota Canals, Francesca Odoardi, Dmitri Lodygin, Alexander Flügel, Viacheslav O. Nikolaev, BjörnPhilipp Diercks, Andreas H. Guse*

Stable Transfection:

Article Title: Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca 2+ microdomains to initiate T cell activation.
Article Snippet: Kai J. Winterberg†, Vanessa Schwentner†, Feng Gu, Franziska Möckl, Gaoyang Li, Andreas Bauche, Stefanie Etzold, Anette Rosche, Mariella Weiß, Nikolaus Thuille, Fritz Förster, Lena Woelk, René Werner, Dejan Kovacevic, Boris Fehse, Roberta Kurelic, Mikolaj Nawrocki, Samuel Huber, HansWilli Mittrücker, Chris Meier, Christa E. Müller, Gottfried Baier, Bjørn S. Skålhegg, Xavier de Deken, Christian WahlSchott, Thomas Mair, Bente Siebels, Roger Cugota Canals, Francesca Odoardi, Dmitri Lodygin, Alexander Flügel, Viacheslav O. Nikolaev, BjörnPhilipp Diercks, Andreas H. Guse*

Transfection:

Article Title: Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca 2+ microdomains to initiate T cell activation.
Article Snippet: Kai J. Winterberg†, Vanessa Schwentner†, Feng Gu, Franziska Möckl, Gaoyang Li, Andreas Bauche, Stefanie Etzold, Anette Rosche, Mariella Weiß, Nikolaus Thuille, Fritz Förster, Lena Woelk, René Werner, Dejan Kovacevic, Boris Fehse, Roberta Kurelic, Mikolaj Nawrocki, Samuel Huber, HansWilli Mittrücker, Chris Meier, Christa E. Müller, Gottfried Baier, Bjørn S. Skålhegg, Xavier de Deken, Christian WahlSchott, Thomas Mair, Bente Siebels, Roger Cugota Canals, Francesca Odoardi, Dmitri Lodygin, Alexander Flügel, Viacheslav O. Nikolaev, BjörnPhilipp Diercks, Andreas H. Guse*



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